A) Explain the experimental results which are seen in the absence of the microsomes.
B) By using the three criteria outlined in the problem decide whether the experimental outcomes in the presence of microsomes (lanes 5 to 8) point out that the protein is translocated across microsomal membranes. Describe the migration of the proteins in lanes 5, 6 and 8.
C. Is the protein anchored in the membrane, or is it translocated all the way via the membrane?