Questions -
1. Think about the advice given in the procedure to test a known oxidase-positive organism along with an unknown.
A) Is this a positive or negative control?
B) What information is provided by the results?
2. Think about the 20-second time limit on the oxidase test.
A) What happens to the oxidase reagent after 20 seconds?
B) Does this "reaction" only happen after 20 seconds? If not why is a 20 second limit set?
3. Provide a possible explanation as to why the phenlenediaminine reagent reduces cytochrome oxidase and not other oxidases.