1. How do uncoupling agents like DNP (dinitrophenol) affect the electron transport chain and the activity of the F0F1 ATP synthase.?
a) They provide an alternative path for protons to re-enter the mitochondrial matrix, so ATP synthesis decreases. Electron flow and proton pumping are not inhibited.
b) They block the flow of electrons, so protons aren't pumped, and ATP synthesis ceases
c) They remove electrons from the chain, so less protons are pumped, and ATP synthesis ceases
d) They block the flow of protons through the ATP synthase, so ATP synthesis ceases. Electron flow and proton pumping are also halted as a result