The standard free energy change of hydrolysis (at 298 K) for acetyl phosphate (shown below) isΔG°′ = - 42.3 kJ/mol.
Acetyl-P + H2O → acetate + Pi
The free energy change for acetyl phosphate hydrolysis in a solution of 2 mM acetate, 2mM phosphate and 3 nM acetyl phosphate is