The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are as follows:
[S] (μmol/L ) |
v [(μmol/L)min-1] |
5 |
22 |
10 |
39 |
20 |
65 |
50 |
102 |
100 |
120 |
200 |
135 |
How would I calculate km and vmax with the information provided?
I created a graph first but I was unsure how to get vmax and km.