Determine the protein concentration in a fraction saved during cytochrome c purification. the Bradford method and used the standard BSA to create a standard curve. 10 µL from the original sample was taken and diluted to a total of 1000 µL total. Using 5 µL from the diluted solution and adding enough water and the dye to bring the total volume up to 3.0 mL.the absorbance value of a solution at 595nm was 0.060. What is the protein concentration (mg/mL) in the original solution? The equation from the BSA standard curve is y=0.0264x+0.0129 where y is the absorbance and x is the concentration in µg/mL