The enzyme catalase catalyzes the decomposition of hydrogen peroxide. The following data are obtained regarding the rate of reaction as a function of substrate concentration.
Run [H2O2]o (M) Initial rate (1/M·s)
1 0.001 0.00138
2 0.002 0.00267
3 0.005 0.00600
The concentration of catalase is 3.5 ×10-9 M.
Use this set of data to determine the maximum rate, Vmax, the Michaelis constant, Km, and the turnover number, k2, for this enzyme.