The diagram below shows the DNA sequence of a gene which is expressed only in the skin of the Jackalope, a diploid eukaryote. Shown is DNA sequence from close to the transcriptional start site, as well as some upstream sequence. The first nucleotide transcribed is an A, and the corresponding A on the coding strand is underlined. Two other sequences in this region are underlined.
Below the sequence is shown the structure of several pieces of DNA that I tested for their ability to initiate transcription. Construct a is the wild-type sequence and b through f either contain small deletions (b-d) or point mutations (e, f). At the right are indicated the relative levels of transcription for each construct, in arbitrary Units.