DENS displays a lifetime near 30ns.
a) Suppose the fundamental anisotropy of DENS is 0.30 and that DENSis bound to a protein with a rotational correlation time of 30ns. What is the anisotropy? "I am calculating that it is just 0.30"
b) Assume now that the protein is bound to an antibody with amolecular weight of 160,000 and a rotational correlation time of100 ns. What is the anisotropy of the DENS-labeledprotein?