Assignment:
A student obtained a silica gel TLC plat shown in figure by spotting samples of Midol, caffeine and acetaminophen on the plate, then eluting with petroleum ether: chloroform
- What are the Rf-values of acetaminophen and caffeine, respectively?
- Based on this TLC analysis, what are the ingredients in a tablet of Midol?
- What are the mobile phase and the stationary phase in this TLC experiment?
- No spots were observed visually when the TLC plate was removed from the developing chamber. How might the student effect visualization of the spots?
- Another student accidentally used Midol PM in her experiment and observed only one spot. Speculate as to which spot was absent and offer a possible explanation for the difference in this student's result.
Provide complete and step by step solution for the question and show calculations and use formulas.