Results of your gram stains


In order for the Gram stain to work properly the ethanol in the decolorizer must be at a concentration greater than 60%. Imagine that you are working in the microbiology laboratory. You are instructed to make more Gram stain reagents. You mistakenly prepare a 50% ethanol solution. How will this affect the results of your Gram stains? Explain. You realize your mistake and prepare the decolorizer using the proper concentration of ethanol. The post-doc in the lab tells you about the Mycoplasma bacteria. These are bacteria that are taxonomically related to the Gram positive bacteria but do not have a cell wall. You are curious and decide to conduct a Gram stain on the Mycoplasma that you have in the lab. What result will you observe? Explain why.

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Biology: Results of your gram stains
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