Restriction digestion reaction with 5 ug dna


How will you set up a 50 ul restriction digestion reaction with 5 ug DNA (with a concentration of 250 ng/ul), 5 units/ ug DNA EcoRI enzyme (10 unit/ ul stock), 1x buffer from 10 x buffer? include all components as needed, calculations and a brief description of how you set this up.

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Biology: Restriction digestion reaction with 5 ug dna
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