Problem- a highly supercoiled DNA molecule is cleaved at one site by a restriction endonuclease, yielding linear DNA. Predict whether each of the following will increaes, decrease, or remain unchanged as a consequence of this treatment.
1) Second virial coefficient
2) Osmotic pressure at 0.1 mg/ml
3) Limit of pi/C as C --> 0
4) Diffusion coefficient
Explain the each and every step in detail for help