Assignment:
When microorganisms reproduce they release waste products that may change the pH and prevent further reproduction. Therefore culture mediums for growing microorganisms are usually buffered. The median for the culture of lactobacilli includes a buffer prepared by dissolving 25 grams K2HPO4 and 25 grams KH2PO4 in water and diluting to 250.0 mL. The question is what is the pH of the buffer solution? Before the culture medium is used it is diluted to 1.000 L with distilled water. So then what would be the pH after dilution as well?
Provide complete and step by step solution for the question and show calculations and use formulas.