Question 1:
A reaction has been designed to be taken out in a 5 ml total volume. It needs a final glycine concentration of 20 µM. If the protocol permits 200µl for the glycine addition, what concentration would you make up in stock solution?
Question 2:
When you added 10 ml of 50mM glucose, 25ml of 100mM glucose and 15ml of 0.1 M NaCl altogether, what would be the final concentration of each component in the solution?
Question 3:
You have 2.5 mg/ml solution of an amino acid (molecular weight = 100). You are needed to add up 0.5 µmoles to a test tube. How much would you pipette?
Question 4:
You require a 10mM solution of a potent and dangerous transcription inhibitor (molecular weight 500) for an experiment. To avoid handling the powder form of this compound you decide to make up the entire bottle instead of attempt to weigh out a portion. The compound comes in 200 mg batches. What volume would you add up to one of such bottles to make up a 10 mM solution?
Question 5:
You have been given with the given solutions of a compound (molecular weight = 500). What volume would you require to pipette to add 10 µmoles of this compound by using these solutions?
a) 40 mM
b) 25mg/dL
c) 0.5%(w/v)
d) 250 mg/ml