Name of enzyme you will use, and source (organism)
The substrate
How you will measure enzyme activity (method)
What type of treatment you will you; type of solution(s), pH, length of exposure, how you will treat your samples
The control(s) in the experiment
Sample size
Maybe how you plan to present your data (table and/or type of graph)
Anything else you would like to get feedback on before you start your experiment.