MES (2-(N-Morpholino) ethanesulfonic acid) is a common buffer utilized in biochemistry labs to stabilize proteins. The pKa for MES is 6.09.
a. Explain what is the pH of 1 liter of a 200mM MES aqueous solution?
b. Explain how much solid NaOH (in grams) requires to be added to the MES solution to make a buffer at pH 6.5?