Mode of inhibition the kinetics of an enzyme are measured, Chemistry

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Mode of inhibition the kinetics of an enzyme are measured

Mode of inhibition. The kinetics of an enzyme are measured as a function of substrate concentration in the presence and absence of 2 mM inhibitor (I).

[S] (µM) Velocity (µmol/min)

No Inhibitor Inhibitor

3 10.4 4.1

5 14.5 6.4

10 22.5 11.3

30 33.8 22.6

90 40.5 33.8

a. What is the binding constant of this inhibitor?

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