Question 1: Give an account on restriction endonucleases and their categorization.
Question 2: Write about the artificial chromosomes BAC and YAC.
Question 3: Give an account on the Gene transfer methods used in genetic engineering.
Question 4: Describe basic principle and variations of PCR.
Question 5: Give an account on the human genome project (HGP).
Question 6: Describe the significance of DNA ligase and reverse transcriptase.
Question 7: Give an account on the viral vectors.
Question 8: What do you mean by the term reported gene assay? Describe with illustrations.
Question 9: Describe the significance of southern and northern blotting methods.
Question 10: Give an account on the applications of genetic engineering in agriculture.