Make the following "working" dilutions of alkaline phosphatase (total 0.4 mL each) in microfuge tubes by diluting the stock preparation of 1.3 microgram/mL alkaline phosphatase. use the enzyme buffer (100 mM Tris (pH 8), 5 mM MgCl2 and 0.10 mg/mL BSA) for preparaing dilution.
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Working AP concentration in microgram/mL
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Volume stock 1.3 microgram/mL alk.phos.
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Enzyme buffer (pH 8)
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0
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0 micro liter
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400 micro liter
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0.065
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0.13
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0.26
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