Problem
Make a BSA standard curve for your BCA Assay. Your BSA stock solution is at a concentration of 3 mg/mL in Tube A. If you take 150uL of Tube A & mix it with 400uL of a buffer solution in Tube B, your resulting BSA concentration in Tube B is ug/mL. If you take 200uL of Tube B & mix it with 175uL of a buffer solution in Tube C, your resulting BSA concentration in Tube C is ug/mL.