Question 1: The difference between the genome of an organism and the epigenome is:
A. The accumulation of mutations in the DNA sequence.
B. The epigenome refers to changes in DNA expression due to changes in DNA packaging.
C. nothing, both terms refer to the genetic content of an organism
Question 2: In an expression plasmid, the origin of replication: _________________
A. allows the bacteria to duplicate the plasmid.
B. Is the position where the base number begins at 1.
C. allows for growth of plasmid containing bacteria in the presence of antibiotics.
D. is responsible for the replication of mRNA.
Question 3: Which of these is not used in DNA replication?
A. DNA polymerase
B. Primer
C. RNA polymerase
D. Helicase
Question 4: Assuming that the genes required for Isoleucine (an amino acid) biosynthesis are contained within an operon. If the cell is grown in the presence of high amounts of isoleucine you would expect to find:
A. The repressor protein released from the operator, allowing transcription.
B. The repressor protein released from the operator, preventing transcription.
C. The repressor protein to bind to the operator, allowing transcription.
D. The repressor protein to bind to the operator, preventing transcription.
Question 5: A codon in the middle of an mRNA strand was mutated from UGU to UGA. What type of mutation is
A. nonsense
B. silent
C. missense
D. frame shift
Question 6: What do UTRs do?
A. They are proteins that regulate transcription.
B. They are proteins that regulate translation.
C. They are regions of the gene that regulate translation.
D. They are regions of the gene that regulate transcription.
Question 7: Which of the following terms refers to a region of a chromosome that has a specifiic function?
A. Genotype
B. Allele
C. Gene
D. DNA
Question 8: Mitochondria in animal cells contain DNA while only chloroplasts in plants contain DNA.
A. False
B. True
Question 9: Replication of each strand occurs in which direction.
A. opposite
B. same
Question 10: The correct order of DNA segments in an operon is:
A. Operator, promoter, structural genes.
B. Promoter, operator, structural genes.
C. Promoter, structural genes, operator.
D. Operator, structural genes, promoter.
Question 11: Which of these is not used in DNA replication?
A. DNA polymerase
B. Helicase
C. Primer
D. RNA polymerase
Question 12: A gene codes for a protein that is 500 amino acids long. Which of the following mutations is likely to be the most deleterious?
A. a nonsense mutation at the fifth codon
B. a missense mutation at the fifth codon
C. a missense mutation at the 490th codon
D. a nonsense mutation at the 490th codon
Question 13: Is the following statement true of prokaryotic cells, eukaryotic cells or both? One gene can encode multiple different proteins (different in amino acid sequence).
A. Prokaryotic
B. Eukaryotic
C. Both prokaryotic and eukaryotic
Question 14: Is the following statement true of prokaryotic cells, eukaryotic cells or both? Multiple genes can be transcribed together into a single mRNA.
A. Prokaryotic
B. Eukaryotic
C. Both prokaryotic and eukaryotic
Question 15: Separation of the original DNA strands is due to the action of:
A. helicase
B. polymerase
C. single stranded binding protein
D. RNA primers.