Problem:
Question 1: I've been told that the maximum number of cycles in PCR is between 20 and 30.
Required:
Is this true, and what are the reasons for this limitation?
Question 2: What is the mechanism? In other words, how does the tRNA know whether the codon is in 5' to 3' direction? I also heard that anything that is translated from 3' to 5' direction is degraded. Is it true?
Could someone explain this to me?