In this course, we have been learning how to demonstrate the involvement and requirement of a protein in a signaling pathway, which often requires specific modulation of the protein.
You can inhibit the function of a gene's products by which of the following approaches:
You may choose more than one answer. Explain your answers.
a) Knock out organism (where the gene has been removed from a transgenic organism)
b. RNAi specific for the genes RNA product
c. blocking antibody directed against the gene's product
d. antagonist drug to the gene's protein product