a. Draw a rate of product formation graph using an acid phosphatase as an example and label each axis.
b. The Km of an enzyme of an enzyme-catalyzed reaction is 7.5 mu M. What substrate concentration will be required to obtain 35% of Vmax for this enzyme?
c. In an acid phosphatase you have determined Ki to be 10.5 mu M for NaF, a non-competitive inhibitor. How much sodium fluoride is required to bring 65% inhibition of the Vmax?