Important physico-chemical properties of biotin include the following:
a) It forms colourless, needle shaped crystals.
b) The vitamin is sparingly soluble in cold water but highly soluble in hot water. The compound is sparingly soluble in alcohol.
c) Biotin forms salts with alkali hydroxides such as NaOH.
d) The compound is photostable.
e) Biotin is destroyed in acid solution.