Problem
A. You have been tasked to perform a CRISPR based knockout of your gene. Identify all candidate sgRNAs which can knockout all isoforms of your gene. Paste the excel spreadsheet with a list below.
B. Annotate in benchling where these candidate sgRNAs are
C. For two of the sgRNAs you have found, design PCR primers which will amplify the target site and produces a product less < 1000 bp
D. Indicate where in the target site the double strand break will happen.`
E. What is the impact on the protein coding sequence if the following NHEJ mutations occur:
• Single base deletion
• Two base deletion
• Three base deletion.
The response must include a reference list. Using Times New Roman 12 pnt font, double-space, one-inch margins, and APA style of writing and citations.