I have two questions regarding this lab its the same bios, Biology

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I have two questions regarding this lab its the same bios

I have two questions regarding this lab? its the same BIOS 2011 Lab 5 PCR the document online

how can this experiment be refined?

i was think may bigger sample size or different genus of ants

two other ways to group these ants to look for difference in infection rates and would you expect them show similar results meaning difference in the rate of infection and WHY???--

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