Hydroxylamine is a mutagen which causes c-to-t transitions


Hydroxylamine is a mutagen which causes C-to-T transitions exclusively. You and a co-worker are studying a strain of yeast which carries a nonsense mutation in a gene you are interested in studying further, so you decide to mutagenize it to isolate a revertant yeast strain. Assuming you want to create an intragenic suppression mutation, would hydroxylamine be a good mutagen to use? What if you wanted to create an intergenic suppression mutation? Explain why in each case.

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