How would you test if the beta cells functioned in vivo


Problem:

Recent work published in Cell (2014) by Pagliuca et al, described a technique to generate many millions of pancreatic beta cells from human pluripotent cells in vitro.

Required:

Question 1: Using this approach, why is it important to generate homogeneous populations of beta cells?

Question 2: What techniques can be used to test for and increase homogeneity in mixed cell populations?

Question 3: The beta cells were expanded as cellular aggregates grown in suspension culture using a bioreactor. Which key features of the growth conditions would need to be optimised to maximize cell viability?

Question 4: How would you test if the beta cells functioned in vivo?

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Biology: How would you test if the beta cells functioned in vivo
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