How would you make up 10 mL of the stock PCR buffer from the following stock solutions?
- 1 M Tris HCl pH 8.3
- 2 M KCl
- 183 mM MgCl2
- 0.12 %(w/v) Gelatin
- 100 mM for each of the dNTPs; dATP, dGTP, dCTP and dTTP
The stock PCR buffer contains:
- 12.2 mM Tris HCl (pH 8.3),
- 61 mM KCl,
- 1.83 mM MgCl2
- 0.0012% (w/v) gelatin,
- 244 μM dNTPs