Problem
I. You have used primary antibodies raised in mice anti-Cyt c (MW 12 kDa) and anti-b-galactosidase (MW 116 kDa) followed by a treatment with a anti-mouse secondary antibody labeled with a infrared-fluorescent molecule. If you did not know the molecular weight of the two detected proteins, could suggest how to specifically detect each protein with antibodies? Explain.
II. Suggest two experiments confirming that the Cyt c you detected is associated with mitochondria. You don't have to give detailed experimental protocols, but you need to indicate controls that needs to be included in your experiment.
III. Could the measure of ATP production be one of these experiments? Explain.