How to determine the concentration of proteins
Problem:
Typically we determine the concentration of proteins using a 280 nm reading. However, it is reasonable to use 205 nm. I was curious about the effectiveness of this method.
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I am performing a gel extraction to purify DNA after a double digest with EcoRI and BamHI. After the gel extraction I need to complete a ligation step before bacterial transformation.
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There have been many times where I have an itch on say my arm and I scratch it, only to feel the scratching elsewhere on my body.
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