You want to load 15 μg of protein in 10 μL into one ofthe 12% polyacrylamide gel well. The protein needs to be in 1Xbuffer and in a total volume of 0.100 ml. You are given a 4.5 mg/mlprotein solution, a 20X sample buffer, and distilled water. How much of each would you mix together to make requiredvolume?