Problem: Now that you know how many cells were transformed and the efficiency, determine the ratio of DNA molecules ended up transforming the cells to the total number of DNA molecules used to transform. The concentration of the pET15b plasmid used for YOUR transformation in this hypothetical question was 450 μg/ml.
Don't forget to consider;
1. How many colonies you observed from the total plated.
2. How many cells were plated on all 4 plates? How many colonies total? Thus how many DNA molecules were able to transform those cells?
3. How much DNA did you add to each transformation? Thus what ratio of DNA molecules from that total number actually transformed cells?
Show how you determined the percentage or ratio of DNA molecules that actually transformed cells below.