Problem:
Question 1: Explain why you need to correct for absorbance at 260 nm in the A280/A260 assay.
Question 2: What is the role of the BCA in the BCA assay? Why do you need to add Cu2+ to your reactions?
Question 3: How is BioRad DC assasy (Lowry assay) similar to BCA assay?
Question 4: How is Lowry assay different from BCA assay?
Question 5: Why do you need to create a BSA standard curve for each assay?
Does anybody know any source of this kind?