Problem:
Please calculate the following:
Question 1: You have a 5L bottle of 5X TAE avilable, but to perform an experiment you need 1l of 1X TAE.
Solve the problem to make the working solution. Show work.
Question 2: If you wish to prepare 1.2%-50%mL of agarose gel, how much agarose will you add to the 50mL of solution? Show work.
Question 3: How much ethidium bromide (15mg/ml) will you add to 50mL of 1X TBE, if you want a final concentration of 0.5ug/ml? Show work conversion.