How can real-time quantitative pcr determine if one copy of, Biology

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How can real-time quantitative pcr determine if one copy of

How can real-time quantitative PCR determine if one copy of a gene is missing?

a. Only one quarter the amount of protein accumulates in the cell
b. The amount of PCR product that accumulates is more than expected
c. The amount of PR product that accumulates is less than expected
d. Twice as much protein accumulates in the cells
e. More SNPs, will accumulate.

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