1. How can PCR be used to identify if a patient has the H1N1 virus?
2. How is a DNA library generated? Why is this the 1st step in whole genome sequencing?
3. How is a cDNA library generated? How is it different?
4. Would you want to do whole genome sequencing from a cDNA library? Explain why or why not?
5. Define: Taq polymerase, degenerate primers, qPCR, Genomic Library, positional cloning, chromosome walking.
6. How does RFLP reveal difference in DNA sequences between individuals?
7. How is AFLP like RFLP? How is it different?
8. What is a microsatellite maker?
9. How is a microsatellite locus defined (what determines specificity)?