How are the primary antibodies generated, Biology

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How are the primary antibodies generated

Problem

YouTube Video: "The Principle of Immunoassays/ ELISA (Enzyme Linked Immunosorbent Assay)".

1) What does ELISA stand for?

2) What are the five (5) types of ELISA that are mentioned in the video?

3) What are two proteins that can be used to block the surface of the well to prevent the antibody from binding directly to the bottom of the well?

4) How are the primary antibodies generated?

5) Give two reasons why Indirect ELISA is advantageous over Direct ELISA.

6) What is the name of the two primary antibodies used in Sandwich ELISA?

7) What are the two most common enzymes used to activate the substrate which leads to color development?

8) Give the name of one substrate that is activated by each of the two commonly used enzymes.

9) What is the purpose of the standard curve?

10) What is the purpose of positive control?

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