Question - From distribution studies, species M and N are known to have distribution constants between water and hexane of 6.01 and 6.20(K=?M?H2O/?H?hex). The two species are to be separated by elution with hexane in a column packed with silica gel containing absorbed water. The ratio VS/VM for the packing is known to be 0.422.
(a) Calculate the retention factor for each of the solutes.
(b) Calculate the selectivity factor.
(c) How many plates will be needed to provide a resolution of 1.5.
(d) How long a column is needed if the plate height of the packing is 2.2×10-3cm.
(e) If a flow rate of 7.10 cm/min is employed, what time will be required to elute the two species?
Please write answer for each section to receive points