Problem:
Question: Calculate
(a) the velocity and
(b) the degree of inhibition of an enzyme-catalyzed reaction in the presence of 6x10^-4 M substate (Km=10^-3 M) and 2.5x10^-4 M non-competitive inhibitor (Ki = 3x10^-5 M). The Vmax = 515 nmoles*L^-1* min^-1
Show the pedigree and your calculation.