Find separation principle in size-exclusion chromatography

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Question 1: What is the dominant species (H₂A, HA^-, A^2-) of a diprotic acid with pK_a1 = 3.5 and pK_a2 = 6.5 at a pH of 4.8?

Question 2: Draw a Jablonski diagram. Describe the difference between the processes leading to fluorescence and phosphorescence.

Question 3: Why is Raman spectroscopy using a visible light-range laser, whereas the energy of the excited vibrations lies in the infrared energy region?

Question 4: X-ray absorption spectroscopy measures the absorption of X-rays by core electrons. What happens to excited core electrons? Where will you find the absorption edge of Mn 1s electrons, compared to that of Ni 1s electrons?

Question 5: Describe the general composition of a chromatography instrument. Give examples for each component for an instrument that allows analyte separation by vapor pressure.

Question 6: What is the separation principle in size-exclusion chromatography? In which order are analyte being eluted?

Question 7: What is the purpose of isoelectric focusing in electrophoresis?

Question 8: You measure a cell potential of -45mV for a WE of Ag/Ag⁺ in a silver ion solution of unknown concentration against a RE of Ag/Ag⁺ with a silver ion concentration of 0.5M. Determine the unknown Ag⁺ concentration.

Question 9: Identify the Miller indices for the plane drawn into the cubic structure below:

Question 10: Given a unit cell parameter a = 4.0Å and an X-ray energy of 3 keV, calculate the diffraction angle θ for the (1 0 0) plane.

Question 11: What kind of light source is used in dynamic light scattering? Which phenomenon leads to a time-dependence in the measurement?

Question 12: Describe the difference between back scattered electrons and secondary electrons. Focus on the mechanism of signal formation and the difference in information that these two signals provide.

Question 13: You need to determine the relative amount of proline in a mixture of amino acids. Which technique do you choose and why?

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