Expresses a constitutively active map kinase


You isolate fibroblasts from a transgenic mouse embryo that expresses a constitutively active MAP kinase. These cells undergo cell differentiation in culture to make cartilage. Fibroblasts from normal mice do not differentiate, but rather divide infrequently, unless they are treated with fibroblast growth factor. When they are treated with FGF they now differentiate into cartilage. You now perturb signaling by injecting into individual cells a function-blocking antibody that inhibits Raf activation. What would you expect the outcome to be in the fibroblasts derived from the transgenic mice and normal mice? Describe what would happen in the presence and absence of FGF for both normal and transgenic cells. 

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Biology: Expresses a constitutively active map kinase
Reference No:- TGS093361

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