Explain why it is important to remove the TLC plate from the development chamber before the solvent front reaches the top edge of the plate? Explain how would the calculation of Rf values be affected if the solvent was allowed to go over the top edge?
What could you change about the experiment to resolve (separate) these two spots next time? Imagine that after developing the TLC plate, you see that two result spots (from the same solution) are overlapping.