a. Explain what is the pH of 1 liter of a 200mM MES aqueous solution? Require to solve a quadratic equation.
b. Explain how much solid NaOH in grams wants to be added to the MES solution to make a buffer at pH 6.5?
MES (2-(N-Morpholino)ethanesulfonic acid) is a common buffer utilized in biochemistry labs to stabilize proteins. The pKa for MES is 6.09.