Explain the Sponge Method?
In the sponge method, sterilized sponge with 45 x 5 cm contact surface and free from antimicrobial agent is used. Aseptically, it is moistened with 10 ml rinse solution and rubbed against the surface to be sampled. It is kept in a sterile plastic bag and analyzed by transferring to a suitable enrichment media for detecting the pathogen or by plating after making the dilution for quantifying the microbiota.