A. Explain what volume of concentrated HCl and what weight of tris (hydroxymethyl) amino methane (as the base) are needed to make the 100 ml of 0.25M buffer, pH 8.0? [pKa of trishydrochloride is 8.0]
B. Explain how to prepare 250ml of 0.6M triethanolamine buffer, pH7.2, from the free amine and concentrated HCl ?(pKa for the amine hydrochloride is 7.8)