explain the composition of blood agarinfusion, Biology

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explain the composition of blood agarinfusion

Explain the Composition of Blood Agar?

Infusion from beef heart - 500 gm

Tryptose - 10.0 gm

Sodium Chloride - 5.0 gm

Agar - 15 gm

Distilled water - 1000 ml

pH - 7.3

Dissolve above ingredients and autoclave. Cool to 45-50°C and add 50 ml of sterile defibrinated blood aseptically. Rotate to mix thoroughly and pour immediately into sterile plates or tubes before solidification.

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