Problem:
I don't need Taq polymerase. Just need normal DNA polymerase. So the main idea is preparation polymerase directly from Lactobacillus without using cloned vector.
My idea is electrophoresis using agarose gel and calculating the isoelectric point of Polymerase based on protein sequence to know where they will migrate after electrophoresis.
Is it possible for this idea ?
Any ideas? Or better yet, pointers to places where this has already been answered?